Lec 3 | MIT 5.301 Chemistry Laboratory Techniques, IAP 2004
TLC – The Basics Thin-layer chromatography is the most commonly used analytical technique in many research labs. This video walks you through every step, from setting up a developing chamber to calculating Rf values. View the complete course: ocw.mit.edu License: Creative Commons BY-NC-SA More information at ocw.mit.edu More courses at ocw.mit.edu
February 7th, 2010 at 8:21 pm
Where is the Advanced TLC Video?
February 7th, 2010 at 9:04 pm
thx a bunch..this video very useful for me…especially for my final project…
February 7th, 2010 at 9:06 pm
The high tuition at MIT is worth every penny for producing great resources like this for their students. Thank you so much MIT. Other universities should follow in your leadership.
February 7th, 2010 at 9:44 pm
Thanks so much!
You really helped me know what I will be doing in lab.
February 7th, 2010 at 10:02 pm
awesomeeeee
February 7th, 2010 at 11:02 pm
Just discovered this channel by accident. Thanks, MIT, I really appreciate the effort on your part .Looks like I know what I’ll be doing for the next couple weeks.
February 7th, 2010 at 11:18 pm
your videos are excellent! very informative and crystal clear. the best explanation I have seen on any website. great work!
February 7th, 2010 at 11:55 pm
Rf value has many uses. For one, it is a direct and precise way to determine the relative polarity difference of two differing substances. Such information also gives one insight as to the intermolecular force strength and such things as boiling point comparisons and viscosity/surface tension.
February 8th, 2010 at 12:32 am
excellent video……does anybody know where we veiw the ADVANCED TLC VIDEO
February 8th, 2010 at 1:20 am
this was so useful, pretty much just wrote my lab report for me. thank you
February 8th, 2010 at 1:52 am
This MIT material is very good indeed and a great public service. Thank you!
February 8th, 2010 at 2:33 am
I tend to agree. Simply running the unknown against A and B separately should work. The only thing I can imagine is that perhaps occasionally compounds in a mixture bind to each other to give strange results. That would have been picked up in this case by the left -hand plate: if for some reason the A spot in the middle disappeared it may have been ‘hidden’ within the B spot. One other way around that I suppose would have been to run a plate with A, B, A+B, and Unknown. Just a guess however!
February 8th, 2010 at 2:39 am
At the end she mentiones an examle to determine what is the unknown compound is made of and she does the cromatography on two different plates. But I think that she could have used one plate, smiply with A, B and the unknown. Than develop it, and with which material (A or B) the unknown is at the same height, the unknown is made of that (if it’s the micture of the two than she would have seen that the unknown material formed two different spots, one in the height of A and one in the height of B)
February 8th, 2010 at 2:57 am
good way to describe the tech!
February 8th, 2010 at 3:18 am
But what is the use of Rf value??
February 8th, 2010 at 3:59 am
thankyou very much. I have found this video very useful to me who haven’t done the experiment. It help me to clear the messy theory in my mind. It is very kind of you, thankyou very much.
February 8th, 2010 at 4:57 am
awesssomme! thannk you
February 8th, 2010 at 5:19 am
Thank you for the video!
February 8th, 2010 at 5:40 am
thanks, helped with my homework!
February 8th, 2010 at 6:27 am
Excellent job. So well done.
February 8th, 2010 at 7:11 am
thank you
February 8th, 2010 at 8:07 am
Excellent video. I’ve learned a lot about tlc. Please make more.
February 8th, 2010 at 8:09 am
Excellent video. I’ve learned a lot about tlc. Please make more.